Agar Protocols
My experiences cultivating mushroom colonies on agar plates and sterilising petri dishes.
Because I needed a reliable source of DNA for sampling, I had to learn how to control the environmental variables for cultures of mushrooms on petri dishes in my lab.
This was a first-time success, and the contamination rate was very low. (1 out of 20 gone bad)
I have learned to spot contaminations early and to transplant the most rhizomorphic parts while keeping to sterile protocols.
I have also tried different mushroom species, cloning directly from the tissue and from dried caps, from which I had collected the spores for later storage.
MAE Recipe #1
The best recipe that has worked for me so far.
- 10g Agar Agar
- 7.5g Malt (note: if liquid candid, add more)
- 500 ml boiling purified water
notes:
- stir between sterilization to disallow sugar to solidify
- sterilization: 20min @ 15psi
If you have an electric cooker like the Tesla Cooker, the pressure might fluctuate, so I go for approx. 30 min total.
Petri Dish Sterilization: 30 min @ 15psi
Agar Agar Sterilization: 30 min @ 15psi