Agar Protocols
My experiences with cultivating mushroom colonies on agar plates, sterilizing & petri dishes.
MAE Recipe #1
The best recipe that has worked for me so far.
- 10g Agar Agar
- 7.5g Malt (note: if liquid candid, add more)
- 500 ml boiling purified water
notes:
- stir between sterilization to disallow sugar to solidify
- sterilization: 20min @ 15psi
If you have electric cooker like Tesla Cooker, pressure might fluctuate so I go for aprox. 30 min total)
Optionaly wait 24-78hours to see if no contaminants are present. source: Mycology Exploration YouTube
Petri Dish Sterilization: 30 min @ 15psi Agar Agar Sterilization: 30 min @ 15psi
10g Agar Agar 7.5g Malt 500 ml H2O
30 min @ 15psi
note: TeslaCooker vysoky tlak: 50 ~ 70 kPa
varka 1:
5 g agar 3.75 g malt 250 ml distiled water
naplnilo 10 misek, sediment, predelani do krabicky a zpet, pro cisty vysledek bud: trepat, nebo sito po provareni do varu
varka 2: 6 g agar 4.5 g malt 300 ml distiled water
scratch, no growth, podezreni: malo cukru, objednano:
Tekuty Sladovy vytazek Obsah sušiny: 80-82% (tj. nadhodnotit mnozstvi o ty procenta)
varka 3:
5g agar 3.75 * 1.2 = 4.5 g candid malt 250 ml distilled water
poured at 50 C
made total 11 plates, nice n clear agar
innoculated 8.6.2023
na varce 3 uspech spory Moravica, sber 20. listopadu
more nutrient agar test:
2 g agar 3 g candid malt extract 100 ml water
not sure, difference might be due to mycelium gen